Sparsity, Parsimony and Data Reduction - Applications across Multi-Dimensional Electron Microscopy

Four-Dimensional Electron Microscopy

Four-dimensional electron microscopy.

The discovery of the electron over a century ago and the realization of its dual character have given birth to one of the two most powerful imaging instruments: the electron microscope. The electron microscope's ability to resolve three-dimensional (3D) structures on the atomic scale is continuing to affect different fields, including materials science and biology. In this Review, we highlight ...

Four-dimensional ultrafast electron microscopy.

Electron microscopy is arguably the most powerful tool for spatial imaging of structures. As such, 2D and 3D microscopies provide static structures with subnanometer and increasingly with angstrom-scale spatial resolution. Here we report the development of 4D ultrafast electron microscopy, whose capability imparts another dimension to imaging in general and to dynamics in particular. We demonst...

Automated multi-model reconstruction from single-particle electron microscopy data.

Biological macromolecules can adopt multiple conformational and compositional states due to structural flexibility and alternative subunit assemblies. This structural heterogeneity poses a major challenge in the study of macromolecular structure using single-particle electron microscopy. We propose a fully automated, unsupervised method for the three-dimensional reconstruction of multiple struc...

Aberration Corrected Electron Microscopy: Prospects and Applications

Aberration correction in the TEM is now relatively well established with more than 20 corrected instruments installed worldwide. This paper will review these instrumental advances and will also describe the underlying theory and computation required to optimise data acquisition and interpret aberration corrected images. Finally the combination of direct electron optical correction and indirect ...